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TY - JOUR AU - Yadav, Ritesh Kumar AU - Sandeep Jain, Amarjit Singh, AU - Pandit, Madhav AU - Dhakal, Rajeev PY - Y2 - 2025/09/23 TI - Physiological studies on Alternaria porri and Stemphylium vesicarium causing purple blotch complex in onion JF - Horticultural Biotechnology Research JA - HBR VL - 3 IS - 0 SE - Research Articles DO - 10.25081/hbr.2017.v3.3392 UR - https://www.updatepublishing.com/journal/index.php/hbr/article/view/3392 SP - 13-21 AB - <p>Effect of different culture media, pH levels and natural substrates on mycelial growth and sporulation of <em>Alternaria porri </em>and <em>Stemphylium vesicarium,</em> incitants of purple blotch complex of onion was investigated. Maximum colony growth of <em>A. porri</em> and <em>S. vesicarium</em> was recorded on oat meal agar and Richard’s agar while, oat meal agar and V8 juice agar recorded the maximum sporulation, respectively. Similarly, pH 5.0 and 6.0 on potato dextrose agar (PDA) recorded the maximum colony growth of <em>A. porri</em> and <em>S. vesicarium</em>, respectively. None of the pH levels on PDA supported the sporulation of <em>A. porri</em> while maximum sporulation of <em>S. vesicarium</em> was recorded on pH 5.0. Onion seed stalks and garlic leaves were found to be the most suitable natural substrates for mass multiplication of <em>A. porri</em> and <em>S. vesicarium</em>, respectively. The present findings are useful for preparation of inoculums required for resistance breeding and fungicidal evaluation against purple blotch complex.</p> ER -