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@article{Sarkar_Rahman_Alam_2022, title={Direct in vitro organogenesis from leaf and internode of Coccinia cordifolia (L.) Cogn.}, volume={8}, url={https://www.updatepublishing.com/journal/index.php/hbr/article/view/7470}, DOI={10.25081/hbr.2022.v8.7470}, abstractNote={The current study was carried out to elucidate a reproducible protocol to develop plants directly from leaf and internodes to facilitate the genetic transformation in Coccinia cordifolia (L.) Cogn., a medicinal plant of the cucurbitaceae family. In vitro grown leaf and internodes were used, which were collected for regenerated shoots from field-grown nodal segments that were sterilized by 0.1% HgCl2 treatment for 6 minutes. The nodal segment cultured on BAP, Kn and BAP, NAA combination where 1.5 mg/L BAP solitary supplement and augmented supplement with 0.1 mg/L NAA was most effective as 80% shoots regenerated with 4.0±0.37 and 2.7±0.45 shoots per culture, respectively. Collected leaf and internode responded 90% at 1.5 mg/L BAP + 0.1 mg/L NAA fortified full-strength MS medium. The highest number of shoots also regenerated in the same combination which were 8.1 ± 0.30 and 10.2±0.40, respectively and found internode as the best explant for direct organogenesis. For root induction, half strength of MS medium supplemented with 0.1 mg/L IBA was found most effective. The highest number of roots regenerated per shoot (6.8±0.10) and root length (2.8±0.20). The successful acclimatization of the in vitro (80%) grown plantlets proved the validity of the developed protocol of using biotechnological techniques for improving the plant.}, journal={Horticultural Biotechnology Research}, author={Sarkar, Md. Taufiqur Rahman and Rahman, Md. Mizanur and Alam, Mohammad Firoz}, year={2022}, month={Feb.}, pages={1–8} }